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cdna microarray experiment  (Incyte corporation)

 
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    Incyte corporation cdna microarray experiment
    Cdna Microarray Experiment, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+microarray+experiment/cdna+microarray/pm15888328-60-5-11
    Average 90 stars, based on 1 article reviews
    cdna microarray experiment - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Hematopoietic malignancies associated with increased Stat5 and Bcl-x(L) expressions in Ink4a/Arf-deficient mice.
    Article Snippet: The INK4a/ARF locus, which encodes the two distinct proteins p16 and p14, is frequently altered in various hematological malignancies as well as in other types of cancers in humans.. In this study, we surveyed tumors that had spontaneously developed in Ink4a/Arfdeficient mice with an inbred FVB/NJ genetic background.. We found that an Ink4a/Arf-deficiency exerted more severe effects on the induction of hematopoietic malignancies in mice with an inbred FVB/NJ genetic background than in mice with a mixed genetic background.

    Article Title: Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction.
    Article Snippet: .. We have reported separately the cDNA microarray experiment (total, 12 336 genes; Incyte Genomics; rat GEM2/3) that showed an increased expression of 48 genes and decreased expression of 14 genes in failing hypertrophied as opposed to compensated hypertrophied hearts.6 The present study is based on the further validation and mechanistic elucidation of the inflammatory and fibrotic responses seen specifically in failing hearts. .. A total of 12 Ren-2 and 4 SD rats were anesthetized with 2% isoflurane, and a blunt 20-gauge needle was placed in the trachea for intubation, which was connected to a volume-cycled rodent respirator (model 683, Harvard Apparatus) on room air with a tidal volume of 2.5 to 3 mL and respiratory rate of 80 breaths/min.

    Expressing:

    Article Title: Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction.
    Article Snippet: .. We have reported separately the cDNA microarray experiment (total, 12 336 genes; Incyte Genomics; rat GEM2/3) that showed an increased expression of 48 genes and decreased expression of 14 genes in failing hypertrophied as opposed to compensated hypertrophied hearts.6 The present study is based on the further validation and mechanistic elucidation of the inflammatory and fibrotic responses seen specifically in failing hearts. .. A total of 12 Ren-2 and 4 SD rats were anesthetized with 2% isoflurane, and a blunt 20-gauge needle was placed in the trachea for intubation, which was connected to a volume-cycled rodent respirator (model 683, Harvard Apparatus) on room air with a tidal volume of 2.5 to 3 mL and respiratory rate of 80 breaths/min.

    Biomarker Discovery:

    Article Title: Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction.
    Article Snippet: .. We have reported separately the cDNA microarray experiment (total, 12 336 genes; Incyte Genomics; rat GEM2/3) that showed an increased expression of 48 genes and decreased expression of 14 genes in failing hypertrophied as opposed to compensated hypertrophied hearts.6 The present study is based on the further validation and mechanistic elucidation of the inflammatory and fibrotic responses seen specifically in failing hearts. .. A total of 12 Ren-2 and 4 SD rats were anesthetized with 2% isoflurane, and a blunt 20-gauge needle was placed in the trachea for intubation, which was connected to a volume-cycled rodent respirator (model 683, Harvard Apparatus) on room air with a tidal volume of 2.5 to 3 mL and respiratory rate of 80 breaths/min.



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    Image Search Results


    The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: The RNA samples prepared for Affymetrix microarray experiment . Upper panel, western blot analysis of CSIG expression in siCSIG and siNC transiently transfected HEK293 cells. Total protein was extracted, and immunoblotting was performed using specific antibodies against CSIG as indicated. GAPDH served as a loading control. Bottom panel, the intactness of the RNA samples was tested using RNA electrophoresis. The three parallel experiments, indicated as 1, 2, and 3, respectively, were performed with the same siRNA.

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Western Blot, Expressing, Transfection, Electrophoresis

    Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: Agreement between microarray and real-time quantitative RT-PCR data . The blue block represent microarray data, the red block represent real-time PCR results. The results are mean ± SEM and the P -values are all <0.05.

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Quantitative RT-PCR, Blocking Assay, Real-time Polymerase Chain Reaction

    Verification of microarray data by quantitative real-time PCR .

    Journal: Frontiers in Endocrinology

    Article Title: Global Characteristics of CSIG-Associated Gene Expression Changes in Human HEK293 Cells and the Implications for CSIG Regulating Cell Proliferation and Senescence

    doi: 10.3389/fendo.2015.00069

    Figure Lengend Snippet: Verification of microarray data by quantitative real-time PCR .

    Article Snippet: Totally, the detection results indicated that the quality of RNA samples we prepared ultimately meets the requirements of Affymetrix cDNA microarray experiment.

    Techniques: Microarray, Real-time Polymerase Chain Reaction, Binding Assay, Activation Assay